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D the colour was developed upon the addition of DTNB - Phosphate - ethanol reagent. The colour was then read immediately at 412 nm and the enzyme activity was determined in M per SH group (MSH) from a standard curve.Determination of total proteins The total protein content was determined according to the method described by Lowry et al. [32]. This method is based on the reaction between the trypto
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Up as compared with the corresponding values of the control. Data presented in tables 3 show that the rats exposed to the two types of vapours encountered a significant decrease in the norepinephrine, dopamine and serotonin contents of the hippocampus as compared with the control group. On other hand, no significant changes were found in norepinephrine and serotonin between the leaded or the unlea
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Zoic acid (DTNB) which is then read at 412 nm. Determination of lipid peroxidation product (malondialdehyde, MDA) in brain tissue homogenate This method is based on the determination of malondialdehyde (MDA), an end product of lipid peroxidation, which reacts with thiobarbituric acid to yield a pink coloured trimethine complex exhibiting a maximum absorbance at 530-532 nm [28]. HPLC determination
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Zoic acid (DTNB) which is then read at 412 nm. Determination of lipid peroxidation product (malondialdehyde, MDA) in brain tissue homogenate This method is based on the determination of malondialdehyde (MDA), an end product of lipid peroxidation, which reacts with thiobarbituric acid to yield a pink coloured trimethine complex exhibiting a maximum absorbance at 530-532 nm [28]. HPLC determination
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G and biting: the attacker springs in the air and comes down on his opponent repeatedly striking at himPage 3 of(page number not for citation purposes)BMC Physiology 2009, 9:http://www.biomedcentral.com/1472-6793/9/based on the reaction of thiocholine, resulting from enzymatic hydrolysis of acylthiocholines with 5, 5'dithiobis-2nitrobenzoic acid (DTNB). However, the original Ellman procedure was f
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G and biting: the attacker springs in the air and comes down on his opponent repeatedly striking at himPage 3 of(page number not for citation purposes)BMC Physiology 2009, 9:http://www.biomedcentral.com/1472-6793/9/based on the reaction of thiocholine, resulting from enzymatic hydrolysis of acylthiocholines with 5, 5'dithiobis-2nitrobenzoic acid (DTNB). However, the original Ellman procedure was f
1
Up as compared with the corresponding values of the control. Data presented in tables 3 show that the rats exposed to the two types of vapours encountered a significant decrease in the norepinephrine, dopamine and serotonin contents of the hippocampus as compared with the control group. On other hand, no significant changes were found in norepinephrine and serotonin between the leaded or the unlea
1
G and biting: the attacker springs in the air and comes down on his opponent repeatedly striking at himPage 3 of(page number not for citation purposes)BMC Physiology 2009, 9:http://www.biomedcentral.com/1472-6793/9/based on the reaction of thiocholine, resulting from enzymatic hydrolysis of acylthiocholines with 5, 5'dithiobis-2nitrobenzoic acid (DTNB). However, the original Ellman procedure was f