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Immediately extracted from the trace elements and lipids by the use of solid phase extraction CHROMABOND column NH2 phase Cat. No. 730031. The sample was then injected directly into an AQUA column 150 54.6 mm 5 C18, purchased from Phenomenex, USA under the following conditions: mobile phase 97/3 20 Mm potassium phosphate, pH 3.0/ methanol, flow rate 1.5 ml/min, UV 270 nm. norepinephrine, dopamine
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Zoic acid (DTNB) which is then read at 412 nm. Determination of lipid peroxidation product (malondialdehyde, MDA) in brain tissue homogenate This method is based on the determination of malondialdehyde (MDA), an end product of lipid peroxidation, which reacts with thiobarbituric acid to yield a pink coloured trimethine complex exhibiting a maximum absorbance at 530-532 nm [28]. HPLC determination
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Zoic acid (DTNB) which is then read at 412 nm. Determination of lipid peroxidation product (malondialdehyde, MDA) in brain tissue homogenate This method is based on the determination of malondialdehyde (MDA), an end product of lipid peroxidation, which reacts with thiobarbituric acid to yield a pink coloured trimethine complex exhibiting a maximum absorbance at 530-532 nm [28]. HPLC determination
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Maintained at the Faculty of Science, Ain Shams University animal unit where housing, handling, and use of animals were strictly in agreement with the regulations and guidelines set by the Faculty's research ethics committee. Gasoline vapour inhalation studies The dynamic system of exposure to gasoline vapours which closely matches exposure to vapour in open air was applied in this study. The dyna
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Maintained at the Faculty of Science, Ain Shams University animal unit where housing, handling, and use of animals were strictly in agreement with the regulations and guidelines set by the Faculty's research ethics committee. Gasoline vapour inhalation studies The dynamic system of exposure to gasoline vapours which closely matches exposure to vapour in open air was applied in this study. The dyna
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Lum were also excised and used for the determination of monoamines only. Due to the limitation of the tissues, six samples only were analyzed for each parameter. The analysis of each individual sample was repeated three times and the average was recorded.A- Aggressive behaviour evaluations The animals were housed in pairs with like-sexed littermates with whom they had lived continuously. The follo
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G and biting: the attacker springs in the air and comes down on his opponent repeatedly striking at himPage 3 of(page number not for citation purposes)BMC Physiology 2009, 9:http://www.biomedcentral.com/1472-6793/9/based on the reaction of thiocholine, resulting from enzymatic hydrolysis of acylthiocholines with 5, 5'dithiobis-2nitrobenzoic acid (DTNB). However, the original Ellman procedure was f
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G and biting: the attacker springs in the air and comes down on his opponent repeatedly striking at himPage 3 of(page number not for citation purposes)BMC Physiology 2009, 9:http://www.biomedcentral.com/1472-6793/9/based on the reaction of thiocholine, resulting from enzymatic hydrolysis of acylthiocholines with 5, 5'dithiobis-2nitrobenzoic acid (DTNB). However, the original Ellman procedure was f